Well, I have paid the price for my sins. None of the mice whose tails I clipped were mutant. Nope. Not one. Zip. Nada. Shunya. All wild-type. Apparently you may need to breed them to F4 or F5 to get the right mutant genotype out. I'm just at F1. I'm never going to graduate! I mean, you mate them. They take 3 weeks to produce litters. You wait 1 week before you can genotype. Then.....you go back to the drawing board. 1 month's work down the drain.
Mouse work sucks.
Giving this matter some serious thought, I guess that the natural selection pressure is pretty high and will always select against mutant, versus wild-type. Nature has its own way of protecting genomes. And its great. It really is. But, if only she would let go for my mice and let me have my mutants, life would be so much easier.
Sigh...back to mating them once again. Its going to be a long wait. Well...atleast the mice are happy, what with all the whoop-de-doo they're getting.
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment